WebLigation protocol, using Roche Rapid Ligation Kit Brief Protocol. Mix: 5x DNA dilution buffer – 2uL Vector (cut and CIP treated) – 0.25-1uL Insert DNA – 0.5-6.5 uL ddH2O – to final volume of 10uL Vortex and quick centrifuge above mixture, then add: 10uL of 2X rapid ligation buffer 1 uL ligase Vortex and quick centrifuge again. WebIllumina® Stranded mRNA Prep, Ligation (16 Samples) A simple, scalable, cost-effective, rapid single-day solution for analyzing the coding transcriptome leveraging as little as 25 ng of total RNA input. Kit contains library prep reagents for 16 samples. Indexes sold separately, compatible only with IDT for Illumina RNA UD indexes, Ligation.
Quick Ligation™ Kit NEB
WebRecover the linearized vector using the QIAquick Gel Extraction Kit (Qiagen), following the manufacturer’s protocol. 3. Set up ligation of the vector to the antigen fragment at a molar ratio of insert:vector of 6–10∶1 (see Note 8). Ligation can be done by the Roche Rapid Ligation Kit (Roche), following the manufacturer’s procedure. 4. Web*The Quick Ligase Reaction Buffer should be thawed and resuspended at room temperature. 2. Gently mix the reaction by pipetting up and down and microfuge briefly. 3. Incubate at … robert antonelle md white plains
T4 DNA Ligase - Thermo Fisher Scientific
WebThe 2x rapid ligation buffer contains a reducing agent that looks like white flakes when thawed. These white flakes must be completely dissolved back into the buffer before use. Keep the ligase enzyme at -20 degrees until just before you are ready to add it, and return it to the freezer promptly. WebThe QIAGEN PCR Cloning procedure is fast and easy — PCR products are simply mixed with pDrive Cloning Vector and Ligation Master Mix and then incubated at 4–16°C for 30 minutes (e.g., in a refrigerator). Special preparation of PCR products is not required. WebThermo Scientific Rapid DNA Ligation Kit enables fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.The kit contains T4 DNA ligase and a specially … robert anton wilson on hunter thompson