Web17 mrt. 2024 · For purification affinity-based chromatin immunoprecipitation and sequencing (ChIP-seq) experiments, 3-5 x10 6 /ml total thymocytes from control BirA and Nfatc1/A-Bio.BirA mice were cultured in complete RPMI (Gibco™, Thermo Fisher Scientific) containing 10 % FBS (Gibco™, Thermo Fisher Scientific) in the presence of TPA (100 … WebIonomycin is a potent and selective calcium ionophore derived from Streptomyces conglobatus (Liu et al.). It is used as a research tool to rapidly raise the intracellular level …
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Web5 dec. 2024 · Establishment of test systems for the detection of IFN-γ protein by ICS and IL-13 mRNA by PrimeFlow RNA™ To expand the chicken toolbox on antibodies suitable for the detection of IFN-γ by ICS, six monoclonal antibodies, initially successfully applied in ELISA [], were investigated.Following stimulation of splenocytes with PMA/ionomycin, four out … WebOmschrijving: Ionomycin (free acid) (from Streptomyces conglobatus), Millipore® Ionomycin ≥98% Leverancier: Thermo Scientific Omschrijving: Ionomycin ≥98% MSDS Certificaten Ionomycin ≥90% Itemnummer: (ICNA0215961101) Leverancier: MP Biomedicals Omschrijving: Free Acid Selective calcium ionophore. UOM: 1 * 1 mg Bekijk … imdb something wild
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Web9 jan. 2024 · Cells were grown until confluency (~63 × 10 5 cell count) and then stimulated to release 5-HT using allyl isothiocyanate (AITC) and ionomycin (Thermo Fisher Scientific). AITC was purchased as an oil-based solution, which was first diluted in DMSO, then diluted further in diH 2 O to result in a 3 mM AITC stock solution in 30% DMSO. Web1 aug. 2024 · Aim: We investigated whether cannabinoids also activate/desensitize two other 'thermo-TRP's', the TRP channels of vanilloid type-3 or -4 (TRPV3 or TRPV4), and if the TRPV-inactive cannabichromene (CBC) modifies the expression of TRPV1-4 channels in the gastrointestinal tract. Methods: WebNext, after the permeabilization at 4 °C for 20 min, 1 × 10 6 cells were incubated with 0.25 μg PerCP/Cy5.5-conjugated anti-mouse CXCR3 (Thermo fisher, Scientific, Pittsburgh, PA, USA), 1 μg APC-conjugated anti-mouse CCR4 (Biolegend), 0.13 μg FITC conjugated anti-mouse CD25 (Proteintech), 0.125 μg PE-conjugated anti-mouse Foxp3 (after … list of ministries bc government