WebFeb 28, 2024 · After removal of the medium, staining with 25 µM DCFH-DA in PBS was performed for 30 minutes (in dark, room temperature [RT]), followed by addition of 100 µL of 1 mM H 2 O 2 in PBS to half of the wells for another 30 minutes (RT, in dark). The staining solution was discarded, cells were washed once with PBS and 100 µL of PBS was added … WebApr 13, 2024 · Then, the cells were stained with DCFH-DA (10 μmol L −1) for 30 min and washed with PBS three times. The stained cells were observed and imaged using an inverted confocal microscope (Leica TCS SP8). Antibacterial activity assessment

Detection of Total Reactive Oxygen Species in Adherent

WebDCFH-DA staining results showed that the H 2 O 2 levels of all poplars were not significantly different under control growth conditions. However, the accumulation of H 2 … WebApr 13, 2024 · DCFH-DA was used as the probe to determine the intracellular ROS generation ability in MCF-7 cells ( Zhang et al., 2014; Karaoğlu et al., 2024 ). MCF-7 cells were seeded in four-well plates at a density of 1.0 × 10 4 cells per dish for 24 h. The cells were co-incubated with CBT-SiPc (3.0 μM) for 10 h. boart longyear limited

Frontiers An Efficient Nitroblue Tetrazolium Staining and Bright ...

WebA. Paige Davis Volk, Jessica G. Moreland, in Methods in Enzymology, 2014 5.2.2 DCFH. DCFH (2,7-dichlorodihydrofluorescein) is the most widely used fluorescent probe for the … WebMeanwhile, the DCFH-DA staining intensities in the LD, MD, and HD groups were significantly lower than that of the NC group (Figure 3C and F, p<0.05). Moreover, the decreased DCFH-DA staining intensities in ovine MII oocytes were positively related to the increased concentrations of Se/CDs supplementation (p<0.05). clifford tornberg