Biofilm proteomics
WebNov 18, 2016 · Biomass Production for Proteomics and Biofilm Growth Conditions. Harvested cells were stored at −80 °C until further use. For biofilm formation, 20-mL cultures supplemented with 0.5 mM sodium pyruvate were inoculated in cultivation flasks in which glass coverslips were inserted to allow adherence. WebJun 12, 2015 · Proteomics, biofilm, and highly regulated hypothetical proteins: a close relationship. Considering that for more than five decades, we thought that the privileged …
Biofilm proteomics
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WebJun 1, 2005 · @article{osti_978041, title = {Community Proteomics of a Natural Microbial Biofilm}, author = {Ram, Rachna J and Verberkmoes, Nathan C and Thelen, Michael P and Tyson, Gene W and Baker, Brett J and Shah, Manesh B and BlakeII, Robert C and Hettich, Robert and Banfield, Jillian F}, abstractNote = {Using genomic and mass spectrometry … WebJul 18, 2024 · Biofilm proteomics refers to the comparative identification of the entire set of proteins expressed by the bacteria in the biofilm matrix under various conditions …
WebJun 15, 2024 · The biofilm development of inoculated sludge under nTiO 2 stress was monitored for a growth cycle of 30 h at concentrations of 0, 5 and 50 mg/L. The biofilm biomass gradually increased over time. Results showed that nTiO 2 treatment promoted biofilm formation to a certain extent rather than inhibiting biofilm development (Fig. 1 … WebAug 7, 2024 · In this study, we investigated the molecular mechanism of AgNPs against multidrug-resistant Pseudomonas aeruginosa by means of anti-biofilm tests, scanning electron microscopy (SEM), and tandem mass tag (TMT)-labeled quantitative proteomics. The results of anti-biofilm tests demonstrated that AgNPs inhibited the formation of P. …
WebMar 1, 2024 · SDS-PAGE protein profiles and distribution of S. epidermidis 1984 in biofilm and planktonic growth states. The total protein was separated on a 12% SDS PAGE gel (A). The arrow indicates the molecular weight marker (140 kDa). The Venn diagram shows the distribution of the proteins identified via proteomics under both growth conditions (B). WebTo our knowledge, this is the first report about differential proteins between biofilm and planktonic M. tuberculosis, which provided the potential antigens for vaccines and target …
WebNov 28, 2024 · Here we apply a meta-proteomics approach to investigate the mechanisms influencing biofilm formation in a model consortium of …
WebAug 7, 2013 · Biofilm formation is an important process in bacterial pathogens and several lines of evidence suggest that in X. a. pv. citri this process is a requirement to achieve maximal virulence since it has a major role in host interactions. In this study, proteomics was used to gain further insights into the functions of biofilms. dawn vs sunrise timedawn vs somogyi effectWebOct 26, 2006 · Here, we report the use of proteomics for the comparative analysis of subcellular fractions obtained from C. albicans biofilm and planktonic cultures, including … gathered leaves crosswordWebMay 27, 2003 · Recent genomic and proteomic studies have identified many of the genes and gene products differentially expressed during biofilm formation, revealing the … dawn vs somogyi phenomenonWebOct 24, 2024 · Here, we report a chemical biology method to selectively label, enrich, and identify proteins expressed by cells within distinct regions of biofilm microcolonies. We … gathered jeansWebApr 11, 2024 · The M382 biofilms used for membrane proteomics were cultured under the same conditions as those used for transcriptomics, as described above, except that four replicates were prepared. gathered leaves annotatedWebApr 15, 2014 · Comparative proteomics of a degP mutant revealed the importance of DegP in the incorporation of nine proteins into OMVs, including ones involved in biofilm matrix formation and various substrates of the type II secretion system. Taken together, these results suggest that DegP plays an important role in determining the content of OMVs … dawn v wilson